Microscopy

Microscopes: There are four different microscopes available in the laboratory which are listed below. Apart from these, there is also a multi-user microscopy laboratory which is available for all Faculty members and graduate students on a per hour cost basis.  The equipment includes an atomic force microscope, a confocal microscope, a transmission electron microscope, Skyscan-1074 microComputer Tomography (in CHS), microtome, cryomicrotome, and embedding counsel.  

i) Nikon TE2000-U Microscope.  This is an inverted microscope fitted with high quality 4, 10, 20, and 40X objective lenses with phase contrast options.  There is also Epi-fluorescence capabilities with different filters such as ultraviolet, blue, and green which enables collection of fluorescent images.  High resolution black and white images can be captured with Hamamatsu CCD Camera with an internal cooler (C4742-95-12ERG).  Low resolution color images can also be captured using digital color camera.  There is also an Image Analysis Software “Sigma Scan Pro” apart from ImageJ, loaded on a dedicated computer with 1tB hard drive. 

ii) EVOSTM AME-i2111 Digital Inverted Microscope.   It is a fully integrated microscope with a digital camera and LCD display.  Since it occupies very less floor space, it is kept in the tissue culture facility and allows easy view, and capture images quickly without the need of transporting cultures to another location.  It has 4x, 10x Ph, 20x Ph Objectives.  Stage has full range of motion in X and Y directions.  Stage is manually controlled.  Image data are stored on a computer connected to the microscope and accessed through Micron software, loaded on a netbook which occupies very little space.  Digital images are then processed for quantification or publication in the computer connected to Nikon microscope.

iii) Olympus Provis AX70 microscope.  This microscope incorporates multiple illuminators (episcopic and diascopic), analyzers, DIC prisms, fluorescence, and phase contrast capabilities.  It has 1.5, 2, 4 and 20X objectives.  The micrography system is the ultimate in sophistication and performance featuring spot measurement, automatic exposure control, and zoom magnification for flexible, easy framing.

iv) Joel JSM-6360 Scanning Electron Microscope.  It is operated under high vacuum.  Up to 3,00,000x magnification is possible with a resolution of 3 nm.  Accelerating voltage come go up to 30 kV.  It is interfaced with a computer which is also used to control the function.